BioTek Gen5 Software for Imaging & Microscopy

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Gen5 software integrates all the essential steps for high-quality image capture, processing, and analysis in a simplified interface. It is easy to define protocols for 2D and 3D sample workflows in both widefield and confocal imaging. Targeted modules enable specific, in-depth analysis across a range of cell imaging applications.

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  • Capture images using several methods including single image capture, montaging, Z-stacking, and more, to visualize greater areas and depths of a sample. These methods are used to capture short- and long-term kinetic workflows, while beaconing allows image-independent capture across multiple wells of a microplate, or across a vessel. Image capture is optimized with image- and laser-based autofocus.
  • Gen5 provides exceptional image-processing capabilities such as background removal, deconvolution, image stitching, plus Z-projection and 3D rendering. Collectively, the image-processing functions facilitate the analysis of complex sample biology across a wide range of applications.
  • The primary cell analysis capabilities in Gen5 enable automated confluence determination and object counting, ranging from whole organisms to subcellular structures (top images). Each can be done using transmitted light or fluorescence imaging channels.
  • The secondary cellular analysis capabilities enable masking of a second area around the primary mask to gain additional information specific to that object (bottom images). This can be critical for assays such as signal translocation, DNA damage, tumor invasion, evaluation of cytoplasmic structures, and others.
  • Software modules available for use with Gen5 enable automated, advanced application-specific analyses. These include neurite outgrowth, spot counting, automatic region of interest identification, single object tracking, and kinetic scratch wound healing assays.
  • Image and data publishing include detailed graphical representations of calculated results can be generated within the Gen5 software, including IC50/EC50 graphs, linear and parallel line analysis, plus scatterplots and histograms. Graphs, data, high-resolution images, and movie files can be exported automatically for publication or other downstream applications.
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At Pegasus, we’re committed to advancing scientific progress by equipping our clients with reliable instruments and expert support, helping them to drive innovation and achieve excellence in their fields.

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